SuperSignal West Dura 持久性化学发光底物的特性包括：
灵敏度：中飞克级
稳定性：室温下，工作溶液可保持稳定 24 h；试剂盒可保持稳定 1 年
兼容性：硝酸纤维素和 PVDF 膜
信号持续时间：24 小时
推荐的一抗浓度：1:1,000 1:50,000 稀释 (0.02 1.0 g/mL)
推荐的二抗浓度：1:50,000 1:250,000 稀释 (4 20 ng/mL)
比较并详细了解 western 印迹化学发光底物
查看所有可用的 HRP 底物
适用于 HRP 的 SuperSignal West Dura 持久性底物经过优化，可实现高灵敏度和长信号持续时间的检测，非常适用于冷却 CCD 相机检测系统。与信号在 30 60 分钟之内下降到几乎无法检测水平的底物不同，SuperSignal West Dura 化学发光底物产生的信号可稳定保持 24 小时，从而实现胶片或相机的多次曝光。
Product comparison
Blots detected using Thermo Scientific SuperSignal West Dura Substrate (top) or Luminata trade; Crescendo Western HRP Substrate (EMD Millipore Corp.) (bottom) at 10 minutes post substrate incubation. Bands are beta-catenin in HeLa cell lysate samples (10 micro;g total protein in first lane; 2-fold dilutions thereafter), probed with rabbit anti-beta-catenin antibody (Part No. RB-1491-PABX) at 1:2000 dilution, followed by goat anti-rabbit HRP antibody (Part No. 31460) at 4ng/mL and exposure to CL-XPosure X-ray film (Part No. 34091).\" data-omni-action=\"View figure\">
Product comparison
Better sensitivity and signal duration with SuperSignal West Dura Substrate
nbsp;A431 cell lysate was diluted in electrophoresis reducing sample buffer. Lane 1 contained 5 micro;g of A431 lysate. Five 1:1 serial dilutions were then prepared and applied at 10 L/well. After electrophoresis, proteins were transferred to nitrocellulose membrane (Part No. 88018) using the Pierce G2 Fast Blotter (Part No. 62288) and Pierce 1-Step Transfer Buffer (Part No. 84731). Membranes were blocked with 5% milk in TBST Buffer. The membranes were incubated with Anti-beta-catenin (Part No. MA1-300) at 0.3 g/mL and then with Goat anti-Mouse Horseradish Peroxidase Conjugate (Part No. 32430) at 20ng/mL. Identical blots were incubated in either SuperSignal West Dura Substrate (Part No. 34076), Clarity trade; ECL Western Substrate (Bio-Rad Laboratories, Inc.) or Immobilon trade; Western Chemiluminescent HRP Substrate (EMD Millipore Corp.) according to respective manufacturer s instructions. Thirty second exposures of the resulting blots were simultaneously acquired on the myECL Imager (Part No. 62236) with the following settings: black 65,296, white 65,535, and gamma 1.0).\" data-omni-action=\"View figure\">
Better sensitivity and signal duration with SuperSignal West Dura Substrate
Obtain mid-femtogram detection with SuperSignal West Dura Substrate
Turbo GFP-His-HA-FLAG was diluted in electrophoresis reducing sample buffer. Lane 1 contained 1,000 fg of Turbo GFP. Seven 1:1 serial dilutions were then prepared and applied at 10 L/well. After electrophoresis, proteins were transferred to PVDF membrane (Part No. 88518), and then the membrane was blocked with SuperBlock in TBST Buffer (Part No. 37536). The membrane was incubated with Biotinylated Anti-His (Part No. MA1-21315-BTIN) at 1 g/mL and then with Streptavidin-Horseradish Peroxidase Conjugate (Part No. 21126) at 1:50,000 dilution. SuperSignal West Dura Substrate (Part No. 34076) was used for detection.\" data-omni-action=\"View figure\">
Obtain mid-femtogram detection with SuperSignal West Dura Substrate
Longer signal duration with SuperSignal West Dura Substrate
Detection of Hsp86 in HeLa cell lysate on nitrocellulose membranes. HeLa cell lysate was diluted in sample buffer and heated to 95 #730;C for 5 minutes. Lane 1 contained 10 g total protein (total 10 L/well). Four 1:1 serial dilutions were then prepared and loaded at 10 L/well (Lanes 2-5). After transfer to nitrocellulose membranes (Part No. 88024), blots were blocked (Part No. 37542), probed with rabbit anti-Hsp 86 antibody (Part No. PA3-013) at 1:2000 dilution, followed by goat anti-rabbit IgG HRP secondary antibody (Part No. 31460) at 6.6ng/mL. Finally, respective blots were incubated with SuperSignal West Dura Substrate (Part No. 34076) or Amersham trade; ECL Prime (GE Healthcare, Part No. RPN2232) per product instructions. At various time-points following substrate incubation, the two blots were imaged using a CCD camera imager. (Identical imaging exposure parameters were used for both blots at each time-point.)\" data-omni-action=\"View figure\">
Longer signal duration with SuperSignal West Dura Substrate
足够用于：2 次微型凝胶印迹；200 cm2 膜
鲁米诺/增强剂，10 mL
稳定过氧化物缓冲液，10 mL
在室温下储存。
